Human Cyclophilin B/PPIB antibody

Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase B, Cphn-2, Cyclophilin B, CYP-20b, CYPB, CYP-S1, OI9, Rotamase B, S-cyclophilin, SCYLP

26- 216aa, 26-216aa, AA408962, AA553318, AI844835, Cphn 2, Cphn2, Cphn-2, Cyclophilin B, Cyclophilin-B, CyclophilinB, CyP 20b, CyP-20b, CyP20b, CYP S1, CYP-S1, CYPS1, CYPB, CYP-S1: Cyclophilin-B, MGC14109, MGC2224, Peptidyl prolyl cis trans isomerase B, Peptidyl prolyl cis trans isomerase B precursor, Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase, PPIB, Rotamase, S cyclophilin, SCYLP, ACB0825-10 µg, ACB0825-20 µg, ACB0825-50 µg, ACB0825-100 µg, ACB0825-250 µg, ACB0825-500 µg, ACB0825-1 mg, ACB0825-010, ACB0825-020, ACB0825-050, ACB0825-100, ACB0825-250, ACB0825-500, ACB0825-01M

Watashi K, et al., (2005) Mol Cell 19 (1) :111-112.; ; Yurchenko V, et al., (2001) Biochem Biophys Res Commun 288 (4) :786-788.; ; E.Roydon P, et al., (1991) Proc Natl Acad Sci USA 88:1903-1907.

Choi JW, et al. Severe osteogenesis imperfecta in cyclophilin B-deficient mice. (PLoS Genet. 2009) {https://pubmed.ncbi.nlm.nih.gov/19997487/}; ; Dubnikov T, et al. PrP-containing aggresomes are cytosolic components of an ER quality control mechanism. (J Cell Sci. 2016) {https://pubmed.ncbi.nlm.nih.gov/27550517/}; ; Mukadam AS, et al. Analysis of novel endosome-to-Golgi retrieval genes reveals a role for PLD3 in regulating endosomal protein sorting and amyloid precursor protein processing. (Cell Mol Life Sci. 2018) {https://pubmed.ncbi.nlm.nih.gov/29368044/}

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

The cell lysates of HepG2 (30ug) and HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2; ; Lane 2.: HeLa The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: A549 cell lysate; ; Lane 5.: MCF7 cell lysate; ; Lane 6.: SK-OV-3 cell lysate; ; Lane 7.: LnCap cell lysate {ACB0825-WB2.jpg} The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Cyclophilin B recombinant protein; ; Lane 2.: Cyclophilin A recombinant protein; ; Lane 3.: Cyclophilin D recombinant protein; ; Lane 4.: Cyclophilin E recombinant protein; ; Lane 5.: Cyclophilin F recombinant protein; ; Lane 6.: Cyclophilin G recombinant protein; ; Lane 7.: Cyclophilin H recombinant protein {ACB0825-WB3.jpg}

Flow cytometry analysis of Cyclophilin B in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained with ACB0825 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .