Human Cyclophilin B/PPIB antibody

Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Cyclophilin B is necessary for the prolactin-induced proliferation, cell growth, and the nuclear retrotransport of prolactin.

Product Specifications

Product Name Alternative

Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase B, Cphn-2, Cyclophilin B, CYP-20b, CYPB, CYP-S1, OI9, Rotamase B, S-cyclophilin, SCYLP

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Cyclophilin B (26-216aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

K2E2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

26- 216aa, 26-216aa, AA408962, AA553318, AI844835, Cphn 2, Cphn2, Cphn-2, Cyclophilin B, Cyclophilin-B, CyclophilinB, CyP 20b, CyP-20b, CyP20b, CYP S1, CYP-S1, CYPS1, CYPB, CYP-S1: Cyclophilin-B, MGC14109, MGC2224, Peptidyl prolyl cis trans isomerase B, Peptidyl prolyl cis trans isomerase B precursor, Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase, PPIB, Rotamase, S cyclophilin, SCYLP, ACB0825-10 µg, ACB0825-20 µg, ACB0825-50 µg, ACB0825-100 µg, ACB0825-250 µg, ACB0825-500 µg, ACB0825-1 mg, ACB0825-010, ACB0825-020, ACB0825-050, ACB0825-100, ACB0825-250, ACB0825-500, ACB0825-01M

References & Citations

Watashi K, et al., (2005) Mol Cell 19 (1) :111-112.; ; Yurchenko V, et al., (2001) Biochem Biophys Res Commun 288 (4) :786-788.; ; E.Roydon P, et al., (1991) Proc Natl Acad Sci USA 88:1903-1907.

Other References

Choi JW, et al. Severe osteogenesis imperfecta in cyclophilin B-deficient mice. (PLoS Genet. 2009) {https://pubmed.ncbi.nlm.nih.gov/19997487/}; ; Dubnikov T, et al. PrP-containing aggresomes are cytosolic components of an ER quality control mechanism. (J Cell Sci. 2016) {https://pubmed.ncbi.nlm.nih.gov/27550517/}; ; Mukadam AS, et al. Analysis of novel endosome-to-Golgi retrieval genes reveals a role for PLD3 in regulating endosomal protein sorting and amyloid precursor protein processing. (Cell Mol Life Sci. 2018) {https://pubmed.ncbi.nlm.nih.gov/29368044/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000933

Uniprot Accession Number

P23284

WB Description

The cell lysates of HepG2 (30ug) and HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2; ; Lane 2.: HeLa The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: A549 cell lysate; ; Lane 5.: MCF7 cell lysate; ; Lane 6.: SK-OV-3 cell lysate; ; Lane 7.: LnCap cell lysate {ACB0825-WB2.jpg} The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Cyclophilin B recombinant protein; ; Lane 2.: Cyclophilin A recombinant protein; ; Lane 3.: Cyclophilin D recombinant protein; ; Lane 4.: Cyclophilin E recombinant protein; ; Lane 5.: Cyclophilin F recombinant protein; ; Lane 6.: Cyclophilin G recombinant protein; ; Lane 7.: Cyclophilin H recombinant protein {ACB0825-WB3.jpg}

FACS Description

Flow cytometry analysis of Cyclophilin B in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained with ACB0825 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .