Human Crystallin alpha B/CRYAB antibody

Alpha-Crystallins are composed of two gene products; alpha-Crystallin A and B, for acidic and basic, respectively. alpha-Crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) . They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-Crystallin A and B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. alpha-Crystallin B is expressed widely in many tissues and organs and occurs in many neurological diseases.

Product Specifications

Product Name Alternative

Crya2, Crya-2, Cryab, Crystallin alpha B, Alpha-crystallin B chain, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Crystallin alpha B (1-175 aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

2E8.

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

AACRYA, Alpha crystallin B chain, Alpha (B) crystallin, Alpha-crystallin B chain, Crya2, Crya-2, Cryab, CRYAB (Crystallin alpha B), Crystallin alpha B, Crystallin alpha polypeptide 2, CTPP2, Heat shock 20 kD like protein, Heat shock protein beta 5, Heat shock protein beta-5, HspB5, NY REN 27 antigen, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, ACB0617-10 µg, ACB0617-20 µg, ACB0617-50 µg, ACB0617-100 µg, ACB0617-250 µg, ACB0617-500 µg, ACB0617-1 mg, ACB0617-010, ACB0617-020, ACB0617-050, ACB0617-100, ACB0617-250, ACB0617-500, ACB0617-01M

References & Citations

Hasan, A., et al. (2002) Biochemistry 41 (52) 15876-15882.; ; Kamradt, M.C., et al (2002) J. Biol. Chem. 277 (41) 38731-38736.; ; Reddy, G.B., et al (2002) FEBS lett. 522 (1-3) 59-64.; ; Ito, H., et al (2002) J. Biochem. 131 (4) 593-603.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_001876.1

Uniprot Accession Number

P02511

WB Description

The recombinant protein (50ng) of crystallin alpha A (alphaA) and the tissue lysates (40ug) of mouse eye (ME) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human crystallin alpha B antibody (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of Crystallin alpha B in HeLa cells. The cell was stained with ACB0617 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .