Human Calmodulin 2/CALM2 antibody

Calmodulin has been known to act as an intracellular calcium sensor protein. When the intracellular Ca2+ concentration increases, calmodulin can bind up to four Ca2+, changing its conformation and regulating cellular functions such as activation or inhibition of a large number of enzymes, ion channels, and receptors.

Product Specifications

Product Name Alternative

PHKD, CAMII, PHKD2, Calmodulin 2, CALM 2, Calmodulin2, CAM II, LP7057 protein, PHKD 2, Phosphorylase kinase delta, Calmodulin-2, CALM2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Calmodulin (1-149aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

J4D8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Calmodulin, ACA0830-10 µg, ACA0830-20 µg, ACA0830-50 µg, ACA0830-100 µg, ACA0830-250 µg, ACA0830-500 µg, ACA0830-1 mg, ACA0830-10, ACA0830-20, ACA0830-50, ACA0830-100, ACA0830-250, ACA0830-500, ACA0830-1

References & Citations

Zhang M., et al. (1998) . Biochem Cell Biol. 76 (2-3) :313-23.; ; James.P., et al. (1995) . Trend Biochem Sci. 20: 38-42.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001734

Uniprot Accession Number

P0DP24

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calmodulin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: NIH3T3 cell lysate ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: Jurkat cell lysate ; ; Lane 4.: A549 cell lysate ; ; Lane 5.: MCF7 cell lysate ; ; Lane 6.: Ramos cell lysate ; ; Lane 7.: PC3 cell lysate

FACS Description

Flow cytometry analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .