Human Crystallin alpha A/CRYAA antibody

Alpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively. alpha-crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP) . They act as molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weight aggregates. These heterogeneous aggregates consist of 30-40 subunits of the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and the participation in the intracellular architecture.

Product Specifications

Product Name Alternative

Acry-1, Alpha crystallin A chain, CRYA1, CRYAA, Crystallin alpha 1, Crystallin alpha A, Heat shock protein beta 4, HSPB 4, HspB4, Zonular Central Nuclear Cataract

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Crystallin alpha A (1-173aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

C9F2

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

A A Crystallin, Acry-1, Alpha crystallin A chain, Alpha-crystallin A chain, CRYA 1, CRYA1, CRYAA, Crystallin Alpha 1, Crystallin alpha A, Heat shock protein beta 4, Heat shock protein beta-4, HSPB 4, HspB4, Zonular Central Nuclear Cataract, ACA0401-10 µg, ACA0401-20 µg, ACA0401-50 µg, ACA0401-100 µg, ACA0401-250 µg, ACA0401-500 µg, ACA0401-1 mg, ACA0401-010, ACA0401-020, ACA0401-050, ACA0401-100, ACA0401-250, ACA0401-500, ACA0401-01M

References & Citations

Fujii N., et al., (2003) Mol. Vis. 9: 915-22.; ; Laksanalamai P., et al., (2004) Extremophiles 8: 1-11.

Other References

Oh JY, et al. Identification of the HSPB4/TLR2/NF-κB axis in macrophage as a therapeutic target for sterile inflammation of the cornea. (EMBO Mol Med. 2012) {https://pubmed.ncbi.nlm.nih.gov/22359280/}; ; Fan Q, et al. Identification of proteins that interact with alpha A-crystallin using a human proteome microarray. (Mol Vis. 2014) {https://pubmed.ncbi.nlm.nih.gov/24453475/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_000385

Uniprot Accession Number

P02489

WB Description

The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. This antibody is not shown cross-activity about Crystallin alpha B.; ; Lane 1.: Recombinant human Crystallin alpha A protein; ; Lane 2.: Recombinant human Crystallin alpha B proteinThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Crystallin alpha A Transfected 293T cell lysate {ACA0401-WB2.jpg}

FACS Description

Flow cytometry analysis of Crystallin alpha A in Balb/3T3 cells. The cell was stained with ACA0401 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .