Human CEBP beta/CEBPB antibody

CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBPalpha, C/EBPbeta, C/EBPdelta, C/EBPepsilon, and C/EBPgamma. Among these proteins, C/EBPbeta is usually highly expressed in liver, intestine, lung and adipose. C/EBPbeta has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP) . These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.

Product Specifications

Product Name Alternative

CCAAT/enhancer-binding protein beta, Liver activator protein, LAP, liver-enriched transcriptional activator protein, Liver-enriched inhibitory protein, LIP, Nuclear factor NF-IL6, NFIL6, Transcription factor 5, TCF-5, Interleukin 6-dependent DNA-binding protein, IL6DBP, CRP2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human C/EBP-beta (1-271 aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

47A1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

EBP-b, ABP0609-10 µg, ABP0609-20 µg, ABP0609-50 µg, ABP0609-100 µg, ABP0609-250 µg, ABP0609-500 µg, ABP0609-1 mg, ABP0609-10, ABP0609-20, ABP0609-50, ABP0609-100, ABP0609-250, ABP0609-500, ABP0609-1

References & Citations

Lekstrom-Himes J. et al. (1998) J Biol. Chem. 273, 28545-28548.; ; Parkin SE. et al. (2002) J Biol. Chem. 277:23563-23572.; ; Su WC. et al. (2003) J Biol. Chem. 278, 51150-51158.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_005185

Uniprot Accession Number

P17676

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C/EBP-beta antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate

IF Description

ICC/IF analysis of C/EBP-beta in HaLa cells. The cell was stained with ABP0609 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .