Human BMP-7 antibody

BMP-7 (bone morphogenetic protein-7), also known as OP-1 (osteogenic protein-1), is a member of the transforming growth factorbeta (TGFbeta) superfamily. BMP-7 plays an essential role during ontogeny of the mammalian eye and kidney, also, is expressed in heart, lung, small intestine, limb bud and teeth. In addition, noggin, one of the soluble BMP antagonists, regulated the action of BMPs on mesenchymal precursor cells, partially through a feedback type of inhibition.

Product Specifications

Product Name Alternative

BMP 7, Bone morphogenetic protein 7, Eptotermin alfa, OP-1, Osteogenic protein 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BMP7 (293-431aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

40000000

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Osteogenic protein1, Osteogenic protein 1, OP-1, OP1, OP 1, Eptotermin alfa, Bone morphogenetic protein 7, BMP 7, BMP7, BMP-7, ABM0615-10 µg, ABM0615-20 µg, ABM0615-50 µg, ABM0615-100 µg, ABM0615-250 µg, ABM0615-500 µg, ABM0615-1 mg, ABM0615-010, ABM0615-020, ABM0615-050, ABM0615-100, ABM0615-250, ABM0615-500, ABM0615-01M

References & Citations

Gregory KE., et al. (2005) J. Biol, Chem. 280, 27970-27980.; ; Sampath Tk., et al. (1990) J. Biol. Chem. 265:13198-13205.; ; Godin RE., et al. (1998) Development. 125:3473-3482.; ; Groppe J., et al. (2002) Nature. 420:636-642.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001710

Uniprot Accession Number

P18075

WB Description

The extracts of mouse kidney (MK) and rat heart (RH) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP7 antibody (1:1,000) . Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of BMP7 in HeLa cells. The cell was stained with ABM0615 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .