Human BID antibody
BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with another Bcl-2 family protein, Bax, leading to the insertion of Bax into the outer mitochondrial membrane. Bax is believed to induce the opening of the mitochondrial voltage-dependent anion channel. This results in the release of cytochrome c and other pro-apoptotic factors from the mitochondria leading to activation of caspases.
Product Specifications
Product Name Alternative
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human BID (1-195aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
4D3
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
References & Citations
Zha J., et al. (2000) Science. 290:1761.; ; Luo X., et al. (1998) Cell. 94:481.; ; Li H., et al. (1998) Cell. 94:491.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
Scientific Category
Apoptosis
NCBI Accession Number
NP_001187
Uniprot Accession Number
P55957
WB Description
IF Description
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