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Human BID antibody

BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with another Bcl-2 family protein, Bax, leading to the insertion of Bax into the outer mitochondrial membrane. Bax is believed to induce the opening of the mitochondrial voltage-dependent anion channel. This results in the release of cytochrome c and other pro-apoptotic factors from the mitochondria leading to activation of caspases.

Product Specifications

Product Name Alternative

BH3 interacting domain death agonist isoform 2, FP497, MGC15319, MGC42355, BH3 interacting domain death agonist isoform 2, BID, BH3 interacting domain death agonist isoform 2 Apoptotic death agonist, Apoptotic death agonist BID, BH3 interacting domain death agonist, HGNC:1050, BH3 interacting domain death agonist p11, BH3 interacting domain death agonist p13, BH3 interacting domain death agonist p15, BID isoform ES (1b), BID isoform L (2), Desmocollin type 4, Human BID coding sequence, OTTHuMP00000196197, p11 BID, p13 BID, p15 BID, p22 BID

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BID (1-195aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

4D3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

BID, ABI0923-10 µg, ABI0923-20 µg, ABI0923-50 µg, ABI0923-100 µg, ABI0923-250 µg, ABI0923-500 µg, ABI0923-1 mg, ABI0923-10, ABI0923-20, ABI0923-50, ABI0923-100, ABI0923-250, ABI0923-500, ABI0923-1

References & Citations

Zha J., et al. (2000) Science. 290:1761.; ; Luo X., et al. (1998) Cell. 94:481.; ; Li H., et al. (1998) Cell. 94:491.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_001187

Uniprot Accession Number

P55957

WB Description

The Jurkat cell lysate (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BID antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BID antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: A549 cell lysate; ; {ABI0923-WB2.jpg}

IF Description

ICC/IF analysis of BID in HeLa cells. The cell was stained with ABI0923 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .
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