Human BHMT antibody

Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.

Product Specifications

Product Name Alternative

Betaine-homocysteine S-methyltransferase 1, BHMT1, Betaine homocysteine methyltransferase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BHMT (1-406aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

3D6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Betaine homocysteine methyltransferase, Betaine homocysteine S methyltransferase, Betaine homocysteine S methyltransferase 1, Betaine-homocysteine methyltransferase, Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase, Betaine-homocysteine S-methyltransferase 1, BHMT, BHMT (Betaine-homocysteine methyltransferase), BHMT1, EC 2.1.1.5, ABH0708-10 µg, ABH0708-20 µg, ABH0708-50 µg, ABH0708-100 µg, ABH0708-250 µg, ABH0708-500 µg, ABH0708-1 mg, ABH0708-010, ABH0708-020, ABH0708-050, ABH0708-100, ABH0708-250, ABH0708-500, ABH0708-01M

References & Citations

Mckeever MP et al., (1991) Clin Sci 81 (4) :551-556; ; Norihiko F et al., (2001) Biochem J 129:313-320; ; Francisco G et al., (2005) Biochem J 391:589-599; ; Catherine M et al., (2005) Biochem J 392:443-448; ; Janet D. S et al., (2006) Biochem J 395:363-371

Other References

Li S, et al. Regulation of homocysteine homeostasis through the transcriptional coactivator PGC-1alpha. (Am J Physiol Endocrinol Metab. 2009) {https://pubmed.ncbi.nlm.nih.gov/19158324/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAH12616

Uniprot Accession Number

Q93088

WB Description

The extracts of mouse liver (each 20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

FACS Description

Flow cytometry analysis of BHMT in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

IF Description

ICC/IF analysis of BHMT in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BHMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .