Human APP/Protease Nexin II antibody

Amyloid precursor protein (APP) is the precursor molecule whose proteolysis generates amyloid beta (AB), a 39- to 42- amino acid peptide and this amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's diseases patients. APP is an integral membrane protein that is phosphorylated in the cytoplasmic and extracellular domains. It has been reported that cell-surface APP plays a role in neurite extension of primary cultured hippocampal neurons. The large extracellular domain of APP is also reported to bind extracellular matrix molecules such as heparin, laminin, and collagen, which can mediate cell adhesion and neurite outgrowth. Abnormal regulation of the metabolism of APP may contribute to the deposition of plaques.

Product Specifications

Product Name Alternative

Amyloid beta A4 protein precursor, isoform, amyloid beta (A4) precursor protein, Alzheimer disease, peptidase nexin-II

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human APP (18-289aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

J4H9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

APP, Protease Nexin II, AAP0836-10 µg, AAP0836-20 µg, AAP0836-50 µg, AAP0836-100 µg, AAP0836-250 µg, AAP0836-500 µg, AAP0836-1 mg, AAP0836-10, AAP0836-20, AAP0836-50, AAP0836-100, AAP0836-250, AAP0836-500, AAP0836-1

References & Citations

Suzuki, T. et al., (1994) EMBO J. 13, 1114-1122.; ; Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_000475

Uniprot Accession Number

P05067

WB Description

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse brain tissue lysate

FACS Description

Flow cytometry analysis of APP/Protease Nexin II in 293T cells. The cell was stained with AAP0836 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of APP/Protease Nexin II in U87MG cells. The cell was stained with AAP0836 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .