Human AK3 antibody

Adenylate kinase (Ak; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2. 7. 4. 3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Ak1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while Ak2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. Ak3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.

Product Specifications

Product Name Alternative

Adenylate kinase, Ak6, FIX, Ak3L1, AkL3L, AkL3L1, adenylate kinase 3, adenylate kinase 6, adenylate kinase 3 like 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Ak3 (adenylaste kinase isozyme 3) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

SJB3-36

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, IHC, FACS

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

AK3, AAK0604-10 µg, AAK0604-20 µg, AAK0604-50 µg, AAK0604-100 µg, AAK0604-250 µg, AAK0604-500 µg, AAK0604-1 mg, AAK0604-10, AAK0604-20, AAK0604-50, AAK0604-100, AAK0604-250, AAK0604-500, AAK0604-1

References & Citations

Noda, L.H. et al. (1973) The Enzymes. Vol. 8, 279-305.; ; Khoo, J.C. et al. (1972) Biochim. Biophys. Acta 268, 98-101.; ; Tomasselli, A.G. et al. (1979) Eur.J.Biochemolecules 93, 257-262.; ; Lee, Y. et al. (1998) J. Biochem. 123, 47-54.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_057366

Uniprot Accession Number

Q9UIJ7

WB Description

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Recombinant Human AK1; ; Lane 2. : Recombinant Human AK2; ; Lane 3. : Recombinant Human AK3 The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: Lncap cell lysate; ; {2004010017118777.jpg}

IHC description

Human liver tissue was incubated with anti-human Ak3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.

FACS Description

Flow cytometry analysis of AK3 in jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of AK3 in U87MG cells. The cell was stained with AAK0604 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .