Human Adiponecticrp30 antibody

Human Adiponectin, also referred to as AdipoQ, Acrp30, apm-1 or GBP28, is a secreted protein expressed exclusively in differentiated adipocyte (an adipokine family member) . Adiponectin contains a modular structure comprising an N-terminal collagenous domain followed by a C-terminal globular domain (gAcrp30) . Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.

Product Specifications

Product Name Alternative

ADIPOQ, ACDC, ACRP30, APM1, GBP28, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human adiponectin (15-244aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

5H7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Adiponectin, Acrp30, AAD0614-10 µg, AAD0614-20 µg, AAD0614-50 µg, AAD0614-100 µg, AAD0614-250 µg, AAD0614-500 µg, AAD0614-1 mg, AAD0614-10, AAD0614-20, AAD0614-50, AAD0614-100, AAD0614-250, AAD0614-500, AAD0614-1

References & Citations

Maeda K. et al., (1996) Biochem Biophys Res Commun. 221:286-289.; ; Berg AH. et al., (2001) Nat Med. 7:947-953.; ; Berg AH. et al., (2002) Trends Endocrinol Metab. 13:84-89.; ; Yamauchi T. et al., (2002) Nat Med. 8:1288-1295.

Other References

Almer G, et al. Adiponectin-coated nanoparticles for enhanced imaging of atherosclerotic plaques. (Int J Nanomedicine. 2011) {https://pubmed.ncbi.nlm.nih.gov/21753879/}; ; Almer G, et al. Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions. (Biologics. 2011) {https://pubmed.ncbi.nlm.nih.gov/22022204/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_004788

Uniprot Accession Number

Q15848

WB Description

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : 293T cell lysate; ; Lane 2. : Adiponectin collagen domain (15-107aa) transfected 293T cell lysate; ; Lane 3. : Adiponectin Full domain (15-244aa) transfected 293T cell lysate; ; Lane 4. : Adiponectin globular domain (108-244aa) transfected 293T cell lysate

IF Description

ICC/IF analysis of Adiponectin in Hep3B cells. The cell was stained with AAD0614 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .