Anti-Vitronectin Antibody
Mouse Monoclonal Antibody specific to Human Vitronectin
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
VN, S-protein, Serum-spreading factor, V75 [Cleaved into: Vitronectin V65 subunit; Vitronectin V10 subunit; Somatomedin-B]
Gene Name
VTN
NCBI Gene ID
<a href="https://www.ncbi.nlm.nih.gov/gene/?term=VTN">VTN</a>
UniProt
P04004
Cellular Locus
Secreted, extracellular space
Host
Mouse
Reactivity
Human
Immunogen
Human Vitronectin
Target Antigen
Vitronectin
Target
Vitronectin
Clonality
Monoclonal
Isotype
IgG1
Type
Antibody
Applications
WB, ELISA, IHC
Field of Research
Cell adhesion
Purification Method
Purified by immunoaffinity chromatography
Dilution
Reconstitute lyophilized antibody in 100ul of distilled water (final concentration 2.0mg/ml).
Format
Purified
Form
Lyophilized
Buffer
Phosphate Buffered Saline
Function
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.; Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Additionnal Information
ELISA: 1:3,000 dilution with solid phase antigen
<br><br>Western Blot: 5-10 ug/ml under reduced and non-reduced conditions.
<br><br>Immunohistochemistry: 5-10 ug/ml on paraffin embedded and frozen tissue sections.
Storage Conditions
The stock solution (2. 0mg/ml) can be stored in aliquots at - 20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored.
Specificity
This antibody specifically reacts with an epitope in the region of amino acids 1- 130 of human vitronectin. It does not react with bovine vitronectin, and this antibody does not interfere with vitronectin-mediated adhesion.
Formulation
10 mM PBS, 1% BSA, pH 7.4, lyophilized
Buffer pH
pH 7.4
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