Anti-Vitronectin Antibody

Mouse Monoclonal Antibody specific to Human Vitronectin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

VN, S-protein, Serum-spreading factor, V75 [Cleaved into: Vitronectin V65 subunit; Vitronectin V10 subunit; Somatomedin-B]

Gene Name

VTN

NCBI Gene ID

UniProt

P04004

Cellular Locus

Secreted, extracellular space

Host

Mouse

Reactivity

Human

Immunogen

Human Vitronectin

Target Antigen

Vitronectin

Target

Vitronectin

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA, IHC

Field of Research

Cell adhesion

Purification Method

Purified by immunoaffinity chromatography

Dilution

Reconstitute lyophilized antibody in 100ul of distilled water (final concentration 2.0mg/ml).

Format

Purified

Form

Lyophilized

Buffer

Phosphate Buffered Saline

Function

Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.; Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

Additionnal Information

ELISA: 1:3,000 dilution with solid phase antigen <br><br>Western Blot: 5-10 ug/ml under reduced and non-reduced conditions. <br><br>Immunohistochemistry: 5-10 ug/ml on paraffin embedded and frozen tissue sections.

Storage Conditions

The stock solution (2. 0mg/ml) can be stored in aliquots at - 20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored.

Specificity

This antibody specifically reacts with an epitope in the region of amino acids 1- 130 of human vitronectin. It does not react with bovine vitronectin, and this antibody does not interfere with vitronectin-mediated adhesion.