Anti-Caspase-10 (CT) Antibody

Rabbit Polyclonal Antibody specific to Caspase-10 (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

CASP-10, EC 3.4.22.63, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE2, ICE-like apoptotic protease 4 [Cleaved into: Caspase-10 subunit p23/17; Caspase-10 subunit p12]

Gene Name

CASP10

Gene ID

843

NCBI Gene ID

UniProt

Q92851

Accession Number

NP_116756

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 505-521 at C-terminus of human FLICE2 (accession no. AAD28402).

Target Antigen

Caspase-10

Target

Caspase-10 (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IF, ICC

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. {PubMed:11717445}.; Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis. {PubMed:17822854}.; Isoform C is proteolytically inactive. {PubMed:11717445}.

Additionnal Information

Immunoblotting: use at 1ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate from HeLa cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes full-length human caspase-10 (59kDa). Since the sequences at C- termini of FLICE2 and Mch4 are different, this antibody recognizes only the FLICE2 form of caspase-10.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4, and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is, therefore, involved in CD95- and TNFR1-induced apoptosis. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8, and -9, thereby causing proteolytic cleavage of many key proteins, such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase- 10 is expressed in many tissues and cell lines.