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E.coli Host Cell Protein ELISA Kit, G3 (6-Strain Combo-K-12, BL21, JM109, DH5α, MC1061, TOP10F)

Product Specifications

Reactivity

E. coli (6-Strain Combo-K-12, BL21, JM109, DH5α, MC1061, TOP10F)

Cross Reactivity

No cross-reaction exists between the homologous antigens.

Type

Sandwich ELISA

Applications

ELISA

Field of Research

ELISA Kit for the quantitative Measurement of host cell protein (HCP) residues in biopharmaceuticals by E. coli (6-Strain Combo-K-12, BL21, JM109, DH5α, MC1061, TOP10F) expression systems

Assay Performance Time

3.5 hours

Sample Type

Cell Culture Supernatants, Protein Purification Process, and End-Product

Detection Range

1.95-125ng/ml

Sensitivity

LOD: 0.122ng/ml; LOQ: 1.95ng/ml

Format

48 Tests

Components

1 Pre-Coated Microplate (Detachable) 2 Standard (Stock Solution) -500μg/mL 3 Detection antibody (100x) 4 TMB Substrates 5 Stop Solution 6 Wash Solution (100x) 7 Diluent Buffer (10x) 8 Plate Sealer 9 Instruction Manual

Shipping Conditions

Gel Packs

Storage Conditions

2-8°C

Inter CV

4.5-10.5 %

Intra CV

3.2-8 %

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