PRK2 Monoclonal Antibody

Catalytic activity:ATP + a protein = ADP + a phosphoprotein., domain:The C1 domain does not bind the diacylglycerol (DAG) ., enzyme regulation:Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids and unsaturated fatty acids. Two specific sites, Thr-816 (activation loop of the kinase domain) and Thr-958 (turn motif), need to be phosphorylated for its full activation., function:Exhibits a preference for highly basic protein substrates., PTM:Activated by limited proteolysis with trypsin., PTM:Autophosphorylated., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily., similarity:Contains 1 AGC-kinase C-terminal domain., similarity:Contains 1 C2 domain., similarity:Contains 1 protein kinase domain., similarity:Contains 3 REM (Hr1) repeats.

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.