Recombinant Human TNF alpha (N-6His)
Product Specifications
Product Name Alternative
Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
Accession Number
P01375
Endotoxin
< 1 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 30-150 pg/ml. (QC verified)
Reconstitution
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
Mol Mass:21.8 KDa; APMol Mass:18 KDa, reducing conditions
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM Histidine, 8 %Trehalose, 0.05%Tween80, pH5.0.
Available Sizes
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