Recombinant Human Laminin521 E8 Protein
Product Specifications
Product Name Alternative
Laminin 521; LN521
Accession Number
O15230&P55268&P11047
Endotoxin
< 0.01 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of H9 Human embryonic stem cells. The ED50 for this effect is 82.37 ng/mL (Regularly tested) .
Reconstitution
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
Mol Mass:86.7&25.8&28.5 KDa; APMol Mass: (28-32) & (38-48) & (100-120) KDa, reducing conditions
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Available Sizes
Frequently Asked Questions
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