Recombinant Human Laminin521 E8 Protein

Product Specifications

Product Name Alternative

Laminin 521; LN521

Accession Number

O15230&P55268&P11047

Endotoxin

< 0.01 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of H9 Human embryonic stem cells. The ED50 for this effect is 82.37 ng/mL (Regularly tested) .

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

Mol Mass:86.7&25.8&28.5 KDa; APMol Mass: (28-32) & (38-48) & (100-120) KDa, reducing conditions

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.