Recombinant Human FGF-4 (153AA)

Product Specifications

Product Name Alternative

Fibroblast growth factor 4; FGF-4; Heparin secretory-transforming protein 1; HST; HST-1; HSTF-1; Heparin-binding growth factor 4; HBGF-4; Transforming protein KS3; FGF4; HST; HSTF1; KS3

Accession Number

P08620

Endotoxin

< 0.01 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

Measured in a cell proliferation assay using MCF7 Human breast cancer cells The ED50 for this effect is 2-20 ng/ml. (QC verified) |Human intestinal organoids (ipsc source) were cultured with Activin A , BMP-4 , EGF , Wnt3a , Noggin , R-spondin 1 and FGF-4. The organoids showed good morphology.

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

Mol Mass:16.9 KDa; APMol Mass:16 KDa, reducing conditions

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.