Recombinant Human IL-1b
Product Specifications
Product Name Alternative
Interleukin-1 beta; Catabolin; IL1F2; IL1B
Accession Number
P01584
Endotoxin
< 0.01 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
Measured by its ability to induce NF-kB signaling in 293-IL1 Res cells. The ED50 for this effect is 20-100 pg/ml. (QC verified)
Reconstitution
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
Mol Mass:17.5 KDa; APMol Mass:17 KDa, reducing conditions
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150 mM NaCl, 3% Trehalose, 0.02% Tween 80, 4% Mannitol, pH 7.4.
Available Sizes
Frequently Asked Questions
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