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Recombinant Human/Mouse/Rat Activin A

Product Specifications

Product Name Alternative

Inhibin beta A chain; INHBA; Activin A

Accession Number

P08476

Endotoxin

< 0.01 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

Measured by its ability to induce SMAD signaling in 293-Activin A Res cells. The ED50 for this effect is 1.3 ng/ml. (Regularly tested) |Human intestinal organoids (ipsc source) were cultured with Activin A , BMP-4 , EGF , Wnt3a , Noggin, R-spondin 1 and FGF-4 . The organoids showed good morphology.

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

Mol Mass:13 KDa; APMol Mass:15 KDa, reducing conditions

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Formulation

Lyophilized from a 0.2 μm filtered solution of 4mM HCl.

Available Sizes

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