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Recombinant Human Noggin

Product Specifications

Product Name Alternative

Noggin; NOG

Accession Number

Q13253

Endotoxin

< 0.01 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 10-100 ng/ml in the presence of 300 ng/mL of Recombinant Human BMP‑4 (QC Verified)

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

Mol Mass:23 KDa; APMol Mass:25-33 KDa, reducing conditions

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, 2mM EDTA, pH 7.4.

Available Sizes

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