Cynomolgus IL-17A / CTLA-8 Protein, His Tag (MALS verified)

Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8), which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also, IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO) .Furthermore, IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 17.0 kDa. The protein migrates as 17-18 kDa,22-25 kDa and 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

XP_005552816.1

Expression Region

Gly 24 - Ala 155

Host

HEK293

Target

IL-17A

Conjugation

Unconjugated

Tag

N-10xHis

Source

Cynomolgus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Cynomolgus IL-17A, His Tag (Cat. No. ILA-C5249) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (MALS verified) with a linear range of 0.2-3 ng/mL (QC tested).