TRPM8 (Extracellular region) Antibody

Product Specifications

Background

The Transient Receptor Potential Melastatin (TRPM) subfamily of cation-permeable channels is ubiquitous in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, some TRPM family members, TRPM6 and TRPM7, possess serine/threonine kinase activity and autophosphorylation. TRPM8 is thermoactivated at mildly cold temperatures (>25oC), and can also be activated by compounds that cause a cooling sensation, such as menthol and icilin. TRPM8 is expressed in trigeminal and dorsal root ganglia neurons where it confers sensitivity to cold in the somatosensory system. In vascular smooth muscle, TRPM8 may alter blood flow by constricting or enlarging blood vessels. TRPM8 is also expressed in normal prostate epithelial cells, as well as overexpressed in several primary tumors including colon, lung, skin, breast, and prostate cancers.

Synonyms

LTrpC6, TRPp8, TRP, TRPM8

Swiss Prot

Q7Z2W7

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

TRPM8 (Extracellular region)

Clonality

Monoclonal

Isotype

IgG1

Clone

M572

Conjugation

Unconjugated

Source

Clone M572 was generated from TRPM8 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM8.

Applications

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

This antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 and MCF-7 cells. The antibody is also recommended for immunocytochemical labeling of cells. This site is well conserved in rat and mouse TRPM8, but has low homology to other TRPM family members.