Syntaphilin (N-terminal region) Antibody

Product Specifications

Background

Synaptic vesicles are organelles situated at the distal terminus of the presynaptic neuron. The exocytosis of these vesicles requires docking at the plasma membrane, priming, and fusion. Fusion is mediated by a complex consisting of membrane components of both the synaptic vesicle and the synaptic plasma membrane. The fusion complex consists of the soluble NSF (N-ethyl-maleimide-sensitive factor), SNAPs (soluble NSF attachment proteins), and receptor proteins (SNAREs) that include synaptobrevin, synaptotagmin, syntaxin, and SNAP-25 (synaptosomal-associated protein of 25kDa) . Syntaxin-1 is a key component of the synaptic vesicle docking/fusion machinery which forms the SNARE complex with SNAP-25 and synaptobrevin. Syntaphilin is a brain-specific membrane-associated protein that can inhibit SNARE complex formation by binding free syntaxin-1.

Synonyms

SNPH

Swiss Prot

O15079

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

Syntaphilin (N-terminal region)

Clonality

Monoclonal

Isotype

IgG1

Clone

M371

Conjugation

Unconjugated

Source

Clone M371 was generated from a sequence corresponding to amino acids in the N-terminal region of human syntaphilin. This sequence has high homology to mouse and rat syntaphilin.

Applications

Purification

Purified by Protein A.

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.