Stat5 (Tyr-694), Phosphospecific Antibody

Product Specifications

Background

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation. Stat5 has been found to be constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFNα in B cells, but only Stat5A is phosphorylated in response to IFNα in HeLa cells.

Synonyms

Signal transducer and activator of transcription 5A, Stat5A

Swiss Prot

P42229

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

Stat5 (Tyr-694)

Clonality

Monoclonal

Isotype

IgG1

Clone

M148

Conjugation

Unconjugated

Source

Clone (M148) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 694 of human Stat5A.

Applications

Purification

Purified by Protein A.

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 92 kDa* band corresponding to Stat5A on SDS-PAGE immunoblots of human A431 cells treated with EGF, but does not detect this band in control cells. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B.