Stat3 (N-terminal region) Antibody

Product Specifications

Background

The stat proteins (Stat1-6) function both as cytoplasmic signal transducers and as activators of transcription in response to cytokines and growth factor receptors. Stat3 is expressed as two variants, Stat3α (86 kDa) and Stat3b (79 kDa) that can differ in expression and activity depending on cell type, activation pathway, and cell maturation stage. Both are activated by phosphorylation at Tyr-705, which induces dimerization, nuclear translocation and DNA binding. Stat3α (86 kDa) transcriptional activation may be regulated by phosphorylation at Ser-727 through the MAPK pathway, while Stat3β lacks this serine site.

Synonyms

Signal transducer and activator of transcription 3

Swiss Prot

P40763

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

Stat3 (N-terminal region)

Clonality

Monoclonal

Isotype

IgG1

Clone

M263

Conjugation

Unconjugated

Source

Clone (M263) was generated from a recombinant protein that included amino acid residues in the N-terminal region of human Stat3.

Applications

WB, IP

Purification

Purified by Protein A.

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects 86 and 79 kDa* bands corresponding to Stat3 isoforms on SDS-PAGE immunoblots of human A431 cells. This sequence has high homology to the conserved regions in rat and mouse Stat3 α and β.