Stat1 (Tyr-701), Phosphospecific Antibody

Product Specifications

Background

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat1 is expressed as two variants of 84 and 91 kDa. Stat1 proteins contain SH2 and SH3 domains, and are components of the interferon-stimulated gene factor 3 (ISGF3) complex. This complex is the primary transcription activator induced by the binding of interferon to its receptors. In response to activation by various cytokines and growth factors, stat1 subunits become phosphorylated at tyrosine 701. This leads to translocation of stat1 to the nucleus, resulting in formation of an active ISGF3 complex. Active ISGF3 modulates the transcription of the interferon-stimulated genes. Thus, phosphorylation of Tyr-701 is critical for gene expression mediated by various cytokines and growth factors.

Synonyms

Stat1a, Stat1b, ISGF3, Stat1alpha, Stat1beta

Swiss Prot

P42224

Modification Site

Tyr-701

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

Stat1 (Tyr-701)

Clonality

Monoclonal

Isotype

IgG1

Clone

M135

Conjugation

Unconjugated

Source

Clone (M135) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 701 of human Stat1.

Applications

Purification

Purified by Protein A.

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects 84 and 91 kDa* stat1 variants on SDS-PAGE immunoblots of human A431 treated with EGF, as well as Jurkat and A431 cells treated with pervanadate. The antibody does not detect these variants in control cells. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse stat1.