SHP1 (C-terminal region) Antibody

Product Specifications

Background

SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKCα and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.

Synonyms

PTP1C, SHPTP1

Swiss Prot

P29350

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

SHP1 (C-terminal region)

Clonality

Monoclonal

Isotype

IgG1

Clone

M160

Conjugation

Unconjugated

Source

Clone (M160) was generated from a recombinant protein containing amino acids in the C-terminal region of human SHP1. This sequence is highly conserved in rat and mouse SHP1.

Applications

WB, IP

Purification

Purified by Protein A.

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.