Shank1 (C-terminal region) Antibody

A variety of anchoring and scaffold proteins that are associated with postsynaptic density (PSD) proteins have been discovered. In particular, PSD-95, GRIP, and Homer have been reported to be anchoring proteins for NMDA, AMPA, and metabotropic glutamate receptors. Shank1 is a synaptic protein that may bridge the NMDA receptor complex and the mGluR receptor complex. The Shank family includes Shank1, Shank2 (ProSAP1), and Shank3 (ProSAP2) . These proteins contain several domains involved in protein-protein interactions. These include ankyrin repeats, an SH3 domain, a PDZ domain, a SAM domain, and a proline-rich region. The PDZ domain of Shank directly interacts with the C-terminal region of GKAP, which can bind to the GK domain of PSD-95 family members. The proline-rich region of Shank directly interacts with the EVH1 domain of Homer. Shank1 knock-out mice have altered PSD protein composition, reduced size of dendritic spines, and smaller PSDs. In addition, these mice have weaker basal synaptic transmission and show increased anxiety-related behavior.

Clone M369 was generated from a sequence corresponding to amino acids in the C-terminal region of rat Shank1. This sequence has high homology to human and mouse Shank1.