PYK2 (Tyr-402), Phosphospecific Antibody

Product Specifications

Background

Protein tyrosine kinase, Pyk2 (CAKb, RAFTK, CADTK), is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) . Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration of various cell types. In T-cells, Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR. Phosphorylation of Tyr-402 is required for the phosphorylation of other tyrosines in Pyk2 and provides a binding site for Fyn SH2 during T-cell activation.

Synonyms

CAKbeta, FAK2

Swiss Prot

Q14289

Modification Site

Tyr-402

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

PYK2 (Tyr-402)

Clonality

Monoclonal

Isotype

IgG1

Clone

M269

Conjugation

Unconjugated

Source

Clone M269 was generated from a phosphopeptide containing amino acids surrounding tyrosine 402 in human PYK2.

Applications

Purification

Purified by Protein A.

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 116 kDa* band corresponding to PYK2 on SDS-PAGE immunoblots of human A431, Jurkat and K562 cells treated with pervanadate, but is not observed in control, untreated cells. This sequence is conserved in rat and mouse PYK2, and has significant homology to the conserved site in FAK.