PYK2 (C-terminal region) Antibody

Product Specifications

Background

Protein tyrosine kinase, Pyk2 (CAKb, RAFTK, CADTK), is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) . Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration of various cell types. In T-cells, Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR. Phosphorylation of Tyr-402 is required for the phosphorylation of other tyrosines in Pyk2 and provides a binding site for Fyn SH2 during T-cell activation.

Synonyms

CAKbeta, FAK2

Swiss Prot

Q14289

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

PYK2 (C-terminal region)

Clonality

Monoclonal

Isotype

IgG1

Clone

M229

Conjugation

Unconjugated

Source

Clone M229 was generated from a recombinant human PYK2 protein that included amino acids residues in the C-terminal region.

Applications

Purification

Purified by Protein A.

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 116 kDa* band corresponding to PYK2 on SDS-PAGE immunoblots of mouse brain tissue. This sequence is conserved in rat and mouse PYK2, and has low homology to other related kinases.