PLCγ1 (N-terminal region) Antibody

Product Specifications

Background

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) . At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulates the activity of PLC. PLCδ is activated by both receptor and nonreceptor tyrosine kinases. PLCγ1 forms a complex with EGF and PDGF receptors, which leads to phosphorylation at tyrosine 771, 783, and 1245. In addition, antigen receptor-induced activation of PLCγ1 leads to phosphorylation at both Tyr-775 and Tyr-783. These two sites are equally important for activation of enzymatic activity.

Synonyms

Phospholipase C gamma1, phosphodiesterase

Swiss Prot

P19174

Host

Mouse

Cross Reactivity

Human, Mouse, Rat, Chicken

Target

PLCγ1 (N-terminal region)

Clonality

Monoclonal

Isotype

IgG1

Clone

M156

Conjugation

Unconjugated

Source

Clone (M156) was generated from a synthetic peptide corresponding to amino acids in the N-terminal region of human PLCγ1.

Applications

WB, IP

Purification

Purified by Protein A.

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 150 kDa* protein in human A431, Hct116, and Jurkat cells, as well as in mouse brain. This sequence is highly conserved in rat and mouse PLCγ1, and has low homology to PLCγ2.