PI3 Kinase, p85 (C-terminal region) Antibody

Product Specifications

Background

Phosphoinositide 3-kinase (PI3K) phosphorylates phosphatidylinositol (PI), PI-4-phosphate and PI-4,5-bisphosphate to catalyze the production of PI-3,4,5-triphosphate. Growth factors and hormones activate PI3K to coordinate various cellular events, such as cell growth, cell cycle entry, cell migration and cell survival. This PI3K activation is reversed by PTEN. In cancers caused by the loss of PTEN activity, PI3K is constituitively active and promotes cell proliferation. PI3Ks are composed of a catalytic subunit and a regulatory subunit. Various isoforms of the catalytic subunit (p110a, p110b and p110d) associate with regulatory subunits (p85a and p85b) with the exception of p110g which interacts with a unique p101 regulatory subunit.

Synonyms

PI3K

Swiss Prot

P27986

Host

Mouse

Cross Reactivity

Human, Mouse, Rat, Chicken

Target

PI3 Kinase, p85 (C-terminal region)

Clonality

Monoclonal

Isotype

IgG2a

Clone

M253

Conjugation

Unconjugated

Source

Clone M253 was generated from a recombinant protein corresponding to amino acids in the C-terminal region of human PI3 Kinase α subunit.

Applications

Purification

Purified by Protein A.

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

This antibody detects an 85 kDa* band corresponding to the p85 subunit of PI3 Kinase in western blots of human A431 and Jurkat cells. This region is homologous to similar regions in rat and mouse PI3 Kinase α subunit.