Phosphoserine/threonine Antibody
Product Specifications
Background
Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.
Synonyms
Phosphoser/thr mAb
Modification Site
PSer/Thr
Host
Mouse
Cross Reactivity
Human, Mouse, Rat
Target
Anti-Phosphoserine/threonine
Clonality
Monoclonal
Isotype
IgG1
Clone
M380A/M380B
Conjugation
Unconjugated
Source
PM3801 Phosphoserine/threonine is a mix of two clones: Clone M380A was generated from a phosphothreonine synthetic peptide (coupled to carrier protein)
Applications
WB, IP
Purification
Purified by Protein A.
Dilution
WB (1:300-5000), IP (1-2ug)
Buffer
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Modification
Phosphorylation
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Product Datasheet
https://www.biossantibodies.com/datasheets/bsm-70605M
Specificity
The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA. Clone M380B was generated.from a phosphoserine synthetic peptide (coupled to carrier protein) .
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