P21/CIP1/WAF1 Antibody

The tumor suppressor protein p21/CIP1/WAF1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression. Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element. Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation.

Cyclin-dependent kinase inhibitor 1 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 MDA-6 p21 CDKN1A CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1

The antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of p21 on SDS-PAGE immunoblots of human endothelial cells (HUVEC), HeLa cells, and Fibroblasts. The antibody is also useful for immunoprecipitation and immunofluorescent labeling in human cells.