JNK1 (C-terminal region) Antibody

Product Specifications

Background

The stress-activated protein kinases (SAPK) or Jun-amino-terminal kinases (JNK) are potently activated by stressors such as UV and gamma radiation. Similar to other MAP Kinases, the core signaling unit is composed of a MAPKKK, usually MEKK1-4 or a mixed lineage kinase (MLK), which phosphorylate and activate MKK4-7, leading to dual phosphorylation and activation of JNK kinases. Rho-GTPases (Rac1 and cdc42) can stimulate MEKKs and MLKs, while MKKs can be activated by a GTPase-independent pathway that involves the germinal center kinase family. There are three JNK genes (JNK1, 2, 3) with further diversification resulting from alternative splicing. Active JNK dimers can translocate to the nucleus to regulate transcription through phosphorylation of c-Jun, ATF-2 and other transcription factors.

Synonyms

SAPK1, p49MAPK, MAPK. Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, Stress-activated protein kinase JNK1, Stress-activated protein kinase 1c (SAPK1c), JNK-46

Swiss Prot

P45983

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

JNK1 (C-terminal region)

Clonality

Monoclonal

Isotype

IgG1

Clone

M267

Conjugation

Unconjugated

Source

Clone M267 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human JNK1.

Applications

WB, IHC

Purification

Purified by Protein A.

Dilution

WB (1:300-5000), IHC ()

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

This antibody detects a 46 kDa* protein corresponding to the apparent molecular mass of JNK1 on SDS-PAGE immunoblots of human A431 and HeLa, as well as rat PC12 cells. This sequence has high homology to rat and mouse JNK1, and has homology to similar regions in JNK2 and JNK3.