Integrin β1 (Extracellular region) Antibody

Product Specifications

Background

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members. Integrin β2/αL is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

Synonyms

Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29, TGB1, FNRB, MDF2, MSK12, ITGB1

Swiss Prot

P05556

Host

Mouse

Cross Reactivity

Human

Target

Integrin β1 (Extracellular region)

Clonality

Monoclonal

Isotype

IgG2a

Clone

M041

Conjugation

Unconjugated

Source

Clone M041 was generated from a proprietary antigen related to the extracellular region of human integrin β1 in complex with integrin α expressed in the NCI-H1915 lung cancer cell line.

Applications

WB, IP

Purification

Purified by Protein G.

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Clone M0411 detects 120-150 kDa* bands corresponding to Integrin β1 on SDS-PAGE immunoblots of native human A431, LNCaP, MCF7, and NCI-H446 cell lysates, as well as human lung tissue. The antibody does not detect the denatured form of Integrin β1. Clone M0411 can be used in western blot, immunocytochemistry, and immunoprecipitation, as well as for ELISA detection when paired with Integrin β1 IM0041 as the capture antibody.