Girdin (C-terminus) Antibody

Product Specifications

Background

Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.

Synonyms

APE, Galpha, vesicle, GIV, Girders actin filament, HkRP1, GRDN, CCDC88A

Swiss Prot

Q3V6T2

Host

Mouse

Cross Reactivity

Human

Target

Girdin (C-terminus)

Clonality

Monoclonal

Isotype

IgG1

Clone

M012

Conjugation

Unconjugated

Source

Clone (M012) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human Girdin.

Applications

WB, IP, IHC

Purification

Purified by Protein G.

Dilution

WB (1:300-5000), IP (1-2ug), IHC ()

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Clone M012 mouse monoclonal antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells and human brain tissue. The antibody also works for immmunoprecipitation, immunohistochemistry, and immunocytochemistry.