FXYD5/Dysadherin (Extracellular region) Antibody

The Na+/K+ ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving Na+ export and K+ import across the plasma membrane. It is composed of a large catalytic α subunit and a membrane-spanning auxiliary β subunit. In humans, the Na+/K+ ATPase is a binary complex of an α subunit that has four isoforms (α1-α4) and a β-subunit that has three isoforms (β1, β2, β3) . In addition, the Na+/K+ ATPase can interact with a group of regulatory subunits, the FXYD type I membrane protein family. This family contains FXYD1-7, and each member contains the conserved F-X-Y-D motif in the trans-membrane domain. FXYD5 (Dysadherin) is expressed in a variety of cells and tissue, as well as upregulated in carcinomas. FXYD5 contains extensive O-glycosylation, and is expressed as molecules that range from 35 to 55 kDa depending on cell type. FXYD5 can reduce E-cadherin mediated cell-cell adhesions, and may be involved in carcinogenesis.

Clone M029 mouse monoclonal antibody detects a 35-55 kDa* band on SDS-PAGE immunoblots of human A431, A549, MeWo, MDA-MB-231 cell, as well as human breast, lung, and skin tissues. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the FXYD5 protein on live cells.