EpCAM (Extracellular region) Antibody

Product Specifications

Background

Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein that has an extracellular domain with one thyroglobulin type-1 domain and a short cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma, but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium. Defects in EpCAM cause hereditary non-polyposis colorectal cancer type 8 (HNPCC8) and diarrhea type 5 (DIAR5) . EpCAM plays a role in embryonic stem cell proliferation and differentiation; it up-regulates the expression of FABP5, MYC, and Cyclin A & Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells and in many types of epithelial carcinomas.

Synonyms

GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, Epitelial glycoprotein, EGP, KS 1/4 antigen, KSA, EGP314, CD326, EpCAM

Swiss Prot

P16422

Host

Mouse

Cross Reactivity

Human

Target

EpCAM (Extracellular region)

Clonality

Monoclonal

Isotype

IgG1

Clone

M042

Conjugation

Unconjugated

Source

Clone (M042) was generated from the extracellular region of human EpCAM.

Applications

WB, IP

Purification

Purified by Protein G.

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Clone M042 mouse monoclonal antibody detects a 35-45 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, H1915, and MCF7 carcinomas. This antibody does not detect denatured EpCAM. The antibody works for native western blot, immunoprecipitation, ELISA, and immunocytochemistry.