Cyclophilin B Antibody

Product Specifications

Background

Cyclophilins are a highly conserved family of peptidylprolyl cis-trans-isomerases (PPI) that are targets of the immunosuppressant drug cyclosporin A. These isomerases have been proposed to aid in protein folding. Cyclophilin B (PPIB) is an ER-localized chaperone protein that associates with type I procollagen, and facilitates procollagen sorting and transport through the secretory compartment. Mutations in the cyclophilin B gene lead to aberrant biosynthesis of type I procollagen, and osteogenesis imperfecta, a disorder characterized by bone fragility. Cyclophilin B has also been implicated as a marker in several cancers, including glioblastomas, neuroblastomas, and gastric cancers. In gastric cancer, Stat3 suppression of miR-520d-5p leads to upregulation of its downstream target cyclophilin B, and enables the growth and survival of these cancer cells.

Synonyms

Peptidyl-prolyl cis-trans isomerase B, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin, SCYLP, PPIB, CYPB

Swiss Prot

P23284

Host

Mouse

Cross Reactivity

Human

Target

Cyclophilin B

Clonality

Monoclonal

Isotype

IgG1

Clone

M019

Conjugation

Unconjugated

Source

Clone M019 was generated from a proprietary antigen related to cyclophilin B from the MCF7 breast cancer cell line.

Applications

WB, IP

Purification

Purified by Protein G.

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Clone M019 detects a 20 kDa* band corresponding to the molecular mass of cyclophilin B on SDS-PAGE immunoblots of human LNCaP, MeWo, MCF7, and MDA-MB-231 cell lysates, as well as full length recombinant cyclophilin B, but does not detect recombinant cyclophilin A. The antibody can be used for western blot, immunoprecipitation, protein ELISA, and immunocytochemistry.