CD44 (Hyaluron Binding Region) Antibody

Product Specifications

Background

Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. CD44 ligands include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions as well as in tumor growth and metastasis. CD44 is overexpressed in many types of cancer; the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.

Synonyms

Epican, ECMR-III, PGP-1, LHR, MDU2, MDU3, MIC4 Heparan sulfate proteoglycan HUTCH-I GP90, CD44

Swiss Prot

P16070

Host

Mouse

Cross Reactivity

Human

Target

CD44 (Hyaluron Binding Region)

Clonality

Monoclonal

Isotype

IgG1

Clone

M591

Conjugation

Unconjugated

Source

Clone M591 was generated from a proprietary antigen related to the hyaluron binding region of human CD44 from the MDA-MB-231 breast cancer cell line.

Applications

ELISA

Purification

Purified by Protein G.

Dilution

ELISA (1:500-1000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Clone M591 detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of denatured and native MDA-MB-231 cell lysates. The antibody also detects the denatured and native form of a recombinant human CD44 protein that includes only the hyaluron binding region. Clone M591 can be used in multiple applications including western blot, immunocytochemical labeling, ELISA, and immunoprecipitation, as well as for detecting CD44 in live, unfixed cells.