C-Cbl (Tyr-700), Phosphospecific Antibody

Product Specifications

Background

C-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells. c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain and a C3HC4 RING finger motif. c-Cbl is rapidly tyrosine phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol 3 kinase (PI3K), Crk and 14-3-3 proteins. In human cancer tissues, c-Cbl is frequently tyrosine phosphorylated in a tumor-specific manner. Src-family kinases primarily phosphorylate c-Cbl at Tyr-700, Tyr-731, and Tyr-774. These sites of phosphorylation provide docking sites for downstream signaling components, such as Grb-2, PI3K, and Fyn.

Synonyms

E3 ubiquitin ligase CBL, RING finger 55

Swiss Prot

P22681

Modification Site

Tyr-700

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

C-Cbl (Tyr-700)

Clonality

Monoclonal

Isotype

IgG1

Clone

M161

Conjugation

Unconjugated

Source

Clone (M161) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 700 in human c-Cbl.

Applications

Purification

Purified by Protein A.

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 120 kDa* protein in human Jurkat cells treated with pervanadate, but not in control cells. This sequence is highly conserved in rat and mouse c-Cbl.