Caspase-8 (C-terminal region) Antibody

Product Specifications

Background

The caspases are a group of cysteine enzymes, which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.

Synonyms

Caspase-8, ced3, NEDD2-like protein, p15, p10

Swiss Prot

Q14790

Host

Mouse

Cross Reactivity

Human

Target

Caspase-8 (C-terminal region)

Clonality

Monoclonal

Isotype

IgG2a

Clone

M382

Conjugation

Unconjugated

Source

Clone (M382) was generated from a recombinant protein containing amino acid residues in the C-terminal region of human Caspase-8.

Applications

Purification

Purified by Protein A.

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 55 kDa* protein corresponding to the molecular weight of Caspase-8 on SDS-PAGE immunoblots of human Jurkat, K562, and A431 cells.