Caspase-3 (p17 subunit) Antibody

Product Specifications

Background

The caspases are a group of cysteine enzymes, which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.

Synonyms

Yama, CPP32, apopain, CASP3

Swiss Prot

P42574

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

Caspase-3 (p17 subunit)

Clonality

Monoclonal

Isotype

IgG1

Clone

31A1067

Conjugation

Unconjugated

Source

Clone 31A1067 was developed against full-length recombinant human Caspase-3.

Applications

WB, IHC

Purification

Purified by Protein G.

Dilution

WB (1:300-5000), IHC ()

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects pro Caspase-3 at 32 kDa* and the large subunit of the cleaved form of Caspase-3 at 14-21 kDa. The antibody can be used for western blot, immunohistochemistry and immuno-electron microscopy. The antibody recognizes an epitope in the large domain subunit (p17) of Caspase-3, and it will recognize pro Caspase-3 and the p17 cleavage fragment.