Atrogin-1 Antibody
Product Specifications
Background
Atrogin-1/Muscle Atrophy F-box (MAFbx) is an E3 ubiquitin ligase that mediates proteolysis events that occur during muscle atrophy. This ATP-dependent ubiquitin-mediated proteolysis occurs in response to a variety of catabolic states in muscle. Atrogin is expressed in heart and skeletal muscle, and is upregulated during muscle atrophy. In addition, Atrogin expression increases in C2C12 myotubes after stimulation with cytokines. Atrogin is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Atrogin interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. Mice deficient in Atrogin are resistant to muscle atrophy.
Synonyms
MAFbx, FBX32, Atrogin
Swiss Prot
Q9CPU7
Host
Rat
Cross Reactivity
Human, Mouse, Rat
Target
Atrogin-1
Clonality
Monoclonal
Clone
M314
Conjugation
Unconjugated
Source
Clone M314 was generated from a synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of mouse Atrogin.
Applications
WB
Purification
Antigen Affinity purification
Dilution
WB (1:300-5000)
Buffer
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Modification
Unmodified
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Product Datasheet
https://www.biossantibodies.com/datasheets/bsm-70353T
Specificity
This antibody detects a full length human recombinant Atrogin 1 on SDS-PAGE immunoblots. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32) .
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