Vimentin Antibody

Product Specifications

Background

Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006) . Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons) . Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008) .

Synonyms

CTRCT30 antibody, Epididymis luminal protein 113 antibody, FLJ36605 antibody, HEL113 antibody, VIM antibody, VIME_HUMAN antibody, Vimentin antibody

Gene ID

7431

Swiss Prot

P08670

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

Vimentin

Clonality

Monoclonal

Isotype

IgG2a

Clone

2D1

Conjugation

Unconjugated

Source

Recombinant human vimentin purified from E. coli.

Applications

WB, IHC

Purification

Purified by Protein G.

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

PBS + 10 mM NaN3.

Modification

Unmodified

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Specificity

Specific for endogenous levels of the ~50 kDa vimentin protein.

Gene ID URL

7431

Predicted Cross Reactivity

Dog, Bovine, Sheep, Horse, Chicken, Cat, Rabbit, Guinea Pig, Goat, Zebrafish, Fish, Hamster, Drosophila, Xenopus, Non-Human Primate, Lizard, Vole, Finch