Synaptojanin 1 Antibody

Product Specifications

Background

Synaptojanin is a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms; one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues (Ramjaun AR & McPherson PS, 1996) . The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down’s syndrome (DS) . It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidylinositol-4,5- bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice (Voronov SV et al., 2008) .

Synonyms

4 antibody, 5-trisphosphate 5-phosphatase 1 antibody, Inositol 5' phosphatase (synaptojanin 1) antibody, Inositol 5' phosphatase antibody, INPP 5G antibody, INPP5G antibody, KIAA0910 antibody, PARK20 antibody, Polyphosphoinositide phosphatase antibody, Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 1 antibody, Synaptic inositol 145 trisphosphate 5 phosphatase 1 antibody, Synaptic inositol-1 antibody, Synaptojanin antibody, Synaptojanin 1 antibody, Synaptojanin 1 polyphosphoinositide phosphatase antibody, Synaptojanin-1 antibody, Synaptojanin1 antibody, Synj 1 antibody, Synj1 antibody, SYNJ1_HUMAN antibody

Gene ID

85238

Swiss Prot

Q62910

Host

Mouse

Cross Reactivity

Rat

Target

Synaptojanin 1

Clonality

Monoclonal

Isotype

IgG1

Clone

5H1

Conjugation

Unconjugated

Source

Recombinant protein from the C-terminal region of rat synaptojanin 1.

Applications

WB, IHC

Purification

Purified by Protein G.

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~145 kDa synaptojanin I protein in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.