Synaptobrevin (VAMP) Antibody

Product Specifications

Background

Synaptobrevin (aka VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles (Deak et al., 2004) . Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive deficits in Alzheimer’s disease (Sze et al., 2000) .

Synonyms

SYB antibody, SYB1 antibody, Synaptobrevin 1 antibody, VAMP antibody, VAMP1 antibody, Vesicle associated membrane protein 1 antibody

Gene ID

6843

Swiss Prot

P23763

Host

Mouse

Cross Reactivity

Human, Mouse, Rat, Bovine, Pig, Rabbit, Hamster

Target

Synaptobrevin (VAMP)

Clonality

Monoclonal

Isotype

IgM

Clone

SP10

Conjugation

Unconjugated

Source

Synaptic immunoprecipitate (crude) from human brain.

Applications

WB, IHC

Purification

IgM Purified culture supernatant

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

Specific for endogenous levels of the ~16 kDa synaptobrevin. The antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections.