Neurofilament NF-M Antibody
Product Specifications
Background
Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1) . NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2) .
Synonyms
150 kDa Neurofilament Medium antibody, 160 kDa neurofilament protein antibody, Medium polypeptide 150kDa antibody, NEF3 antibody, Neurofilament 3 antibody, Neurofilament 3 medium antibody, Neurofilament medium polypeptide antibody, Neurofilament protein M antibody, Neurofilament triplet M protein antibody, Neurofilament-3 (150 kD medium) antibody, NF-M antibody, NFM antibody
Gene ID
24588
Swiss Prot
P12839
Host
Mouse
Cross Reactivity
Human, Mouse, Rat, Bovine, Chicken
Target
Neurofilament NF-M
Clonality
Monoclonal
Isotype
IgG1
Clone
3H11
Conjugation
Unconjugated
Source
Preparation containing the extreme C-terminus of rat NF-M, expressed in and purified from E. Coli.
Applications
WB, IHC
Purification
Purified by Protein G.
Concentration
2 mg/ml
Dilution
WB (1:300-5000), IHC ()
Buffer
PBS + 10 mM NaN3.
Modification
Unmodified
Storage Conditions
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Specificity
Specific for endogenous levels of the ~145 kDa neurofilament M protein.
Gene ID URL
24588
Curated Selection
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